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Transversus abdominis plane block in rats: Preliminary cadaveric studies
Chris S Burrows1,2, Juliet C Duncan1, Fernando Martinez-Taboada3
1Royal (Dick) School of Veterinary Studies, University of Edinburgh, UK.
Laboratory Animals
|September 29, 2022
Summary
Ultrasound-guided transversus abdominis plane (TAP) blocks in rat cadavers showed low success rates. While a two-point injection technique may offer greater spread than a single point, current methods are not recommended for clinical use.
Area of Science:
- Veterinary Anesthesiology
- Surgical Research
- Regional Anesthesia Techniques
Background:
- Transversus abdominis plane (TAP) blocks are ultrasound-guided regional anesthesia techniques for abdominal wall analgesia.
- Previous studies in humans and cats show TAP blocks reduce pain and opioid use post-abdominal surgery.
- TAP blocks have not been previously described in rats, and optimal techniques remain debated.
Purpose of the Study:
- To assess the feasibility of ultrasound-guided TAP blocks in rat cadavers.
- To compare the spread of injectate between single-point and two-point injection techniques for TAP blocks in rats.
Main Methods:
- Ultrasound-guided transversus abdominis plane (TAP) blocks were performed on 23 Sprague Dawley rat cadavers.
- A total volume of 1 ml/kg of methylene blue solution was used for injections.
- Injectate spread was measured for both single-point and two-point injection techniques.
Main Results:
- The overall success rate for performing TAP blocks was low, at 21.7%.
- The area of spread for single-point injections was 87.8 ± 32 mm².
- The two-point injection technique resulted in a larger spread area of 102.4 ± 17 mm².
Conclusions:
- The current technique for ultrasound-guided TAP blocks in rat cadavers is not recommended due to a low success rate.
- The two-point injection technique may provide a greater spread of injectate compared to the single-point technique.
- Further research is needed to draw definitive conclusions and refine the technique for reliable coverage.

