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Updated: Aug 27, 2025
Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
Published on: September 20, 2021
STREAMING-Tag System: Technology to Enable Visualization of Transcriptional Activity and Subnuclear Localization of
Hiroaki Ohishi1, Hiroshi Ochiai2
1Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan.
Abstract:
The Spliced TetO REpeAt, MS2 repeat, and INtein sandwiched reporter Gene tag (STREAMING-tag) system enables imaging of nuclear localization as well as the transcription activity of a specific endogenous gene at sub-100-nm resolution in living cells. The use of this system combined with imaging of epigenome states enables a detailed analysis of the impact of epigenome status on transcriptional dynamics. In this chapter, we describe a method for quantifying distances between Nanog gene and clusters of cofactor BRD4 using the STREAMING-tag system in mouse embryonic stem cells.
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