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Updated: Aug 27, 2025

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
Published on: July 28, 2023
SSNIP-seq: A simple and rapid method for isolation of single-sperm nucleic acid for high-throughput sequencing
Stevan Novakovic1, Vanessa Tsui1,2, Tim Semple3
1DNA Repair and Recombination Laboratory, St Vincent's Institute of Medical Research, Melbourne, Victoria, Australia.
Abstract:
We developed a simple and reliable method for the isolation of haploid nuclei from fresh and frozen testes. The described protocol uses readily available reagents in combination with flow cytometry to separate haploid and diploid nuclei. The protocol can be completed within 1 hour and the resulting individual haploid nuclei have intact morphology. The isolated nuclei are suitable for library preparation for high-throughput DNA and RNA sequencing using bulk or single nuclei. The protocol was optimised with mouse testes and we anticipate that it can be applied for the isolation of mature sperm from other mammals including humans.
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