Development of Isospora suis from pigs in primary porcine and bovine cell cultures
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Veterinary Parasitology
|May 1, 1987
Abstract:
Sporozoites of Isospora suis penetrated and developed by endodyogeny in primary porcine kidney (PPK) and primary fetal bovine kidney (PFBK) cell cultures. Motile merozoites and binucleate Type I meronts were observed in both types of cultured cells. Multinucleate Type II meronts developed in PPK cell cultures only. These multinucleate meronts were always found singly, were nonmotile and did not form merozoites.
Insights
Isospora suis sporozoites developed in cell cultures, forming motile merozoites and Type I meronts in both porcine and bovine kidney cells. Unique Type II meronts developed only in porcine cells, showing distinct nonmotile characteristics.
Area of Science:
- Veterinary Parasitology
- Cell Biology
- Infectious Diseases
Background:
- Isospora suis is a significant pathogen in young pigs, causing significant economic losses in the swine industry.
- Understanding the in vitro development of Isospora suis is crucial for developing effective control strategies.
Purpose of the Study:
- To investigate the in vitro developmental stages of Isospora suis in primary cell cultures.
- To compare the development of Isospora suis in primary porcine kidney (PPK) and primary fetal bovine kidney (PFBK) cell cultures.
Main Methods:
- Sporozoites of Isospora suis were inoculated into PPK and PFBK cell cultures.
- Parasite development was monitored using light microscopy.
- Morphological characteristics of developmental stages were documented.
Main Results:
- Sporozoites successfully invaded and developed via endodyogeny in both PPK and PFBK cells.
- Motile merozoites and binucleate Type I meronts were observed in both cell types.
- Multinucleate Type II meronts developed exclusively in PPK cells, appearing singly and nonmotile, without merozoite formation.
Conclusions:
- Primary kidney cell cultures provide a suitable in vitro model for studying Isospora suis development.
- Distinct developmental pathways, including the formation of Type II meronts, may occur in different host cell environments.
- Further research is needed to elucidate the biological significance of Type II meronts in Isospora suis infections.