Colorimetric determination of peroxide value in vegetable oils using a paper based analytical device

Area of Science:

• Analytical Chemistry
• Food Science
• Materials Science

Background:

• Peroxide value (PV) is a critical indicator of lipid oxidation in oils.
• Traditional methods for PV determination can be time-consuming and require laboratory equipment.
• There is a need for rapid, accessible methods for monitoring oil quality.

Purpose of the Study:

• To develop a simple paper-based analytical device (PAD) for the quantitative determination of peroxide value (PV) in vegetable oils.
• To establish the analytical performance and reliability of the developed PAD.
• To provide a cost-effective and user-friendly alternative for on-site oil quality assessment.

Main Methods:

• Development of a two-layered paper-based analytical device (PAD) utilizing iodometric titration principles.
• Reaction of hydroperoxides in vegetable oil with iodide ions to generate iodine on the paper substrate.
• Colorimetric detection of iodine intensity correlated with PV using an inverse logarithmic calibration curve against the official ISO 3960:2017 method.

Main Results:

• The PAD demonstrated a working range of 0.01–30.0 meq/Kg for peroxide value determination.
• A limit of detection of 0.015 meq/Kg was achieved, indicating high sensitivity for edible oils.
• A well-defined inverse logarithmic calibration curve confirmed the correlation between PAD color intensity and PV.

Conclusions:

• The developed paper-based analytical device provides a sensitive, simple, and low-cost method for determining peroxide value in vegetable oils.
• The PAD enables on-site, visual detection of lipid oxidation, facilitating rapid quality control.
• This approach offers advantages such as reduced solvent consumption and ease of use compared to conventional methods.