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FGF21 at physiological concentrations regulates vascular endothelial cell function through multiple pathways.

Ni Yang1, Yucong Zhang1, Yi Huang1

  • 1Department of Geriatrics, Institute of Gerontology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Biochimica Et Biophysica Acta. Molecular Basis of Disease
|September 29, 2022
PubMed
Summary
This summary is machine-generated.

Fibroblast growth factor 21 (FGF21) within physiological levels benefits vascular endothelial cells (VECs) by enhancing metabolism and reducing oxidative stress. This protective effect may involve SIRT1 regulation, highlighting FGF21

Keywords:
Endothelial functionFGF21-regulated geneFibroblast growth factor 21Human umbilical vascular endothelial cellsTranscriptome sequencing

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Area of Science:

  • Cardiovascular Biology
  • Endothelial Cell Function
  • Metabolic Signaling

Background:

  • Cardiovascular diseases link to vascular endothelial cell (VEC) dysfunction.
  • Fibroblast growth factor 21 (FGF21) influences VECs, but its specific effects are unclear.

Purpose of the Study:

  • Assess physiological serum FGF21 concentrations in healthy individuals.
  • Investigate FGF21's effects on human umbilical VECs (HUVECs) and underlying mechanisms.

Main Methods:

  • Cross-sectional study of 212 healthy individuals.
  • In vitro treatment of HUVECs with recombinant FGF21.
  • Assays for glycolysis, nitric oxide, and reactive oxygen species.
  • RNA transcriptomic sequencing and SIRT1 knockdown experiments.

Main Results:

  • Serum FGF21 correlated positively with age and pulse wave velocity.
  • FGF21 (400 pg/mL) enhanced glycolysis, increased nitric oxide, and reduced oxidative damage in HUVECs.
  • Gene expression analysis revealed FGF21 impacts metabolic, inflammation, and apoptosis pathways.
  • SIRT1 appears involved in FGF21's gene regulatory effects.

Conclusions:

  • Physiological FGF21 levels exert beneficial effects on HUVECs.
  • FGF21 enhances endothelial cell metabolism and offers protection against oxidative stress.
  • SIRT1 may mediate some of FGF21's regulatory functions in VECs.