Enzymatic Substrates and Fluorescence Imaging of Influenza Virus Sialidase

Immunostaining with an antiviral antibody is usually performed to visualize virus-infected cells. In contrast, this study established an easy method for fluorescence (FL) imaging of cells infected with influenza A and B viruses and some paramyxoviruses without the need for cell fixation and an antiviral antibody. These viruses and the cells they have infected express the viral surface enzymes "neuraminidase" or "hemagglutinin-neuraminidase," which show sialidase activity. Sialidase activity is fluorescently visualized by using a sialidase fluorogenic probe developed in our previous study. The probe enables histochemical FL imaging of the virus-infected cells and applies to virus isolation and detection of an influenza virus resistant to sialidase inhibitors anti-influenza drugs.