Related Experiment Video
Updated: Aug 27, 2025

Automatic Separation and Collection of Cancer-Related Substances from Clinical Samples
Published on: January 13, 2023
Effective Separation of Cancer-Derived Exosomes in Biological Samples for Liquid Biopsy: Classic Strategies and
Yujiao Xie1,2,3,4, Xiawei Xu1,2,3,4, Jie Lin1,2
1Cixi Institute of Biomedical Engineering International Cooperation Base of Biomedical Materials Technology and Application Chinese Academy of Science (CAS) Key Laboratory of Magnetic Materials and Devices and Zhejiang Engineering Research Center for Biomedical Materials Ningbo Institute of Materials Technology and Engineering CAS Ningbo 315201 P. R. China.
Abstract:
Liquid biopsy has remarkably facilitated clinical diagnosis and surveillance of cancer via employing a non-invasive way to detect cancer-derived components, such as circulating tumor DNA and circulating tumor cells from biological fluid samples. The cancer-derived exosomes, which are nano-sized vesicles secreted by cancer cells have been investigated in liquid biopsy as their important roles in intracellular communication and disease development have been revealed. Given the challenges posed by the complicated humoral microenvironment, which contains a variety of different cells and macromolecular substances in addition to the exosomes, it has attracted a large amount of attention to effectively isolate exosomes from collected samples. In this review, the authors aim to analyze classic strategies for separation of cancer-derived exosomes, giving an extensive discussion of advantages and limitations of these methods. Furthermore, the innovative multi-strategy methods to realize efficient isolation of cancer-derived exosomes in practical applications are also presented. Additionally, the possible development trends of exosome separation in to the future is discussed in this review.
More Related Videos
Related Concept Videos
Overview Of Cell Separation And Isolation
Overview of Exosomes
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

