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Updated: Aug 27, 2025

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
Published on: April 24, 2021
siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells
Aurea Susana Blancas-Velazquez1, Martin Fredensborg Rath2
1Department of Neuroscience, University of Copenhagen, Copenhagen, Denmark.
Abstract:
Suspension primary cultures of rat pineal cells have been used for decades to determine biochemical regulatory mechanisms of pineal melatonin synthesis, but more recently, RNA interference technology has made the study of the role of specific genes in this melatonin-proficient model system possible. We here present a protocol for preparing rat pineal cell cultures and efficiently knock down gene expression by use of synthetic siRNA.
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