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Published on: April 2, 2018
Analysis of the Human Pineal Proteome by Mass Spectrometry
Mariette Matondo1, Guillaume Dumas2,3, Erik Maronde4
1Institut Pasteur, Université de Paris, CNRS USR2000, Proteomics Platform, Mass Spectrometry for Biology Unit, Paris, France.
Abstract:
The human pineal gland regulates the day-night dynamics of multiple physiological processes, especially through the secretion of melatonin. Recently, using mass spectrometry-based proteomics and dedicated analysis tools, we have identified regulated proteins and signaling pathways that differ between day and night and/or between control and autistic pineal glands. This large-scale proteomic approach is the method of choice to study proteins in a biological system globally. This chapter proposes a protocol for large-scale analysis of the pineal gland proteome.
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