Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

13.2K
The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
13.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Melatonin Facts: Musing With the Concept "Melatonin Travels Freely Through Biological Membranes".

Journal of pineal research·2026
Same author

Octadecaneuropeptide, ODN, Promotes Cell Survival against 6-OHDA-Induced Oxidative Stress and Apoptosis by Modulating the Expression of miR-34b, miR-29a, and miR-21in Cultured Astrocytes.

Cells·2024
Same author

Industrial and academic approaches to the search for alternative melatonin receptor ligands: An historical survey.

Journal of pineal research·2024
Same author

Melatonin facts: Melatonin lacks immuno-inflammation boosting capacities at the molecular and cellular levels.

Biochimie·2024
Same author

Polymorphisms and Pharmacogenomics of <i>NQO2</i>: The Past and the Future.

Genes·2024
Same author

Melatonin facts: Lack of evidence that melatonin is a radical scavenger in living systems.

Journal of pineal research·2023

Related Experiment Video

Updated: Aug 27, 2025

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
19:05

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

12.4K

Measuring Binding at the Putative Melatonin Receptor MT3.

Céline Legros1, Philippe Dupuis1, Gilles Ferry2

  • 1Eurofins, Discovery, Eurofins Cerep, Celle-Levescault, France.

Methods in Molecular Biology (Clifton, N.J.)
|September 30, 2022
PubMed
Summary

Melatonin, a neurohormone with antioxidant properties, was long thought to bind to a specific brain receptor called MT3. Research confirmed MT3 is actually the enzyme quinone reductase 2 (NQO2).

Keywords:
2-iodoMCA-NATBindingBrainFast kineticsMelatoninNQO2

More Related Videos

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
08:33

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Published on: March 11, 2021

2.0K
Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
10:13

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Published on: June 9, 2017

16.5K

Related Experiment Videos

Last Updated: Aug 27, 2025

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
19:05

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

12.4K
Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
08:33

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Published on: March 11, 2021

2.0K
Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
10:13

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Published on: June 9, 2017

16.5K

Area of Science:

  • Neuroendocrinology
  • Biochemistry
  • Pharmacology

Background:

  • Melatonin (N-acetyl-5-methoxytryptamine) is a key neurohormone known for its diverse biological functions, including significant antioxidant properties.
  • A putative melatonin binding site, designated MT3, was previously identified in mammalian brains, suggesting a specific receptor interaction.
  • The precise molecular identity of the MT3 binding site remained elusive, prompting extensive research to clarify its nature.

Purpose of the Study:

  • To elucidate the molecular identity of the MT3 binding site, a long-sought target for melatonin.
  • To describe the unique experimental techniques employed in the binding assays used to characterize MT3.
  • To provide a comprehensive overview of the research confirming the nature of the MT3 binding site.

Main Methods:

  • Utilized specialized binding assays to investigate the interaction of melatonin with the putative MT3 site.
  • Employed independent verification studies across multiple research groups to confirm experimental findings.
  • Characterized the biochemical properties of the binding site to determine its enzymatic or receptor nature.

Main Results:

  • The MT3 binding site was consistently identified and confirmed by several independent research groups.
  • Binding studies revealed that MT3 is not a classical receptor but rather possesses enzymatic activity.
  • It was definitively established that the MT3 binding site is the enzyme quinone reductase 2 (NQO2).

Conclusions:

  • The long-standing hypothesis of a distinct MT3 melatonin receptor has been disproven.
  • The enzyme quinone reductase 2 (NQO2) is the molecular entity responsible for the previously identified MT3 binding activity.
  • This finding clarifies the molecular target of melatonin's interaction at the MT3 site, with implications for understanding its biological effects.