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Related Concept Videos

Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

65
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
65

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Plant Polysaccharide Array for Studying Carbohydrate-Binding Proteins.

Anna V Nikiforova1, Victoria V Golovchenko2, Polina V Mikshina3

  • 1Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997, Russia. nikiforovaalica@gmail.com.

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Summary

This study developed a glycoarray to analyze plant carbohydrate-binding proteins (CBP). Researchers discovered ricin

Keywords:
INRA-RU2anti-glycan human antibodiescarbohydrate-binding proteinsglycoarraypectinsplant lectinsplant polysaccharidespolysaccharide chip

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Area of Science:

  • Plant biochemistry
  • Glycobiology
  • Protein-carbohydrate interactions

Background:

  • Many plant carbohydrate-binding proteins (CBP) lack characterized specificity, hindering functional understanding.
  • Bioinformatic analysis identifies numerous potential CBPs, but experimental validation is limited.
  • Understanding CBP specificity is crucial for deciphering plant biological processes.

Purpose of the Study:

  • To develop a novel glycoarray for comprehensive analysis of plant CBP specificity.
  • To investigate the carbohydrate-binding properties of plant polysaccharides and lectins.
  • To explore potential interactions with human antibodies.

Main Methods:

  • Chemical immobilization of 113 plant polysaccharides and 23 mono-/oligosaccharides onto N-hydroxysuccinimide activated surfaces.
  • Creation of a glycan array including ligands for known plant lectins.
  • Validation of immobilization by testing interactions with antibodies and the plant lectin ricin.

Main Results:

  • Functional activity of immobilized plant polysaccharides was preserved.
  • A novel polysaccharide-binding ability of ricin was identified, expanding its known specificity.
  • A diverse repertoire of antibodies against plant polysaccharides was detected in human peripheral blood.

Conclusions:

  • The developed glycoarray is effective for studying plant CBP specificity.
  • Ricin exhibits previously unrecognized interactions with plant polysaccharides.
  • Human blood contains antibodies capable of recognizing plant-derived glycans.