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Updated: Aug 16, 2026
Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
Published on: January 9, 2019
Proliferative potential of meningiomas determined with the monoclonal antibody Ki-67
Abstract:
In 30 meningiomas we investigated the proliferation rate of various subtypes with the monoclonal antibody Ki-67. Frozen sections were incubated with Ki-67 antibody using a modified Alkaline Phosphatase anti-Alkaline Phosphatase (APAAP)-technique and evaluation of proliferation rate was done by cell counting. Meningiomas of the meningiotheliomatous, fibrous and angioblastic subtype without atypical histological findings contained 1% or less proliferating cells. In recurrent tumors, in transitional and in anaplastic meningiomas there is a marked increase of proliferating cells up to 20%. The distribution of marked cells varies in recurrent tumors and anaplastic meningiomas, and a focal proliferation of tumor cells was seen in meningiomas from transitional type. Immunohistological labelling of proliferating cells in meningiomas may allow a more precise prediction of the proliferation potential of each meningioma.
Insights
Ki-67 antibody immunohistochemistry reveals varying proliferation rates in meningioma subtypes. Higher proliferation indicates potential for recurrence and anaplastic changes, aiding in prognosis.
Area of Science:
- Neuropathology
- Oncology
- Immunohistochemistry
Background:
- Meningiomas are the most common primary intracranial tumors.
- Accurate prediction of meningioma behavior is crucial for patient management.
- Proliferation rate is a key indicator of tumor aggressiveness.
Purpose of the Study:
- To investigate the proliferation rate of different meningioma subtypes using Ki-67.
- To correlate proliferation rates with histological subtypes and clinical behavior (recurrence, anaplasia).
- To evaluate the utility of Ki-67 immunohistochemistry for predicting meningioma potential.
Main Methods:
- Studied 30 meningioma samples.
- Utilized Ki-67 monoclonal antibody with a modified Alkaline Phosphatase anti-Alkaline Phosphatase (APAAP) technique on frozen sections.
- Quantified proliferation rate via cell counting.
Main Results:
- Meningiomas without atypical features (meningiotheliomatous, fibrous, angioblastic) showed ≤1% Ki-67 positive cells.
- Recurrent, transitional, and anaplastic meningiomas exhibited significantly increased proliferation (up to 20%).
- Focal proliferation was observed in transitional meningiomas; distribution varied in recurrent and anaplastic types.
Conclusions:
- Ki-67 immunohistochemistry effectively differentiates meningioma subtypes based on proliferation.
- Elevated Ki-67 labeling is associated with aggressive behavior, including recurrence and anaplasia.
- Ki-67 staining offers a valuable tool for predicting meningioma proliferation potential and prognosis.
