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Circ-CTNNB1 drives aerobic glycolysis and osteosarcoma progression via m6A modification through interacting with
Feng Yang1, Yangyang Liu2, Jun Xiao1
1Department of Orthopedic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
Cell Proliferation
|October 1, 2022
Summary
Circular RNA circ-CTNNB1 promotes osteosarcoma (OS) growth and metastasis by enhancing glycolysis. This occurs through interaction with RBM15 and N6-methyladenosine (m6A) modification, driving OS progression.
Area of Science:
- Oncology
- Molecular Biology
- Epigenetics
Background:
- Circular RNAs (circRNAs) are noncoding RNAs involved in cancer development.
- circ-CTNNB1 has been implicated in various cancers, but its role in osteosarcoma (OS) is unclear.
- Osteosarcoma is a highly malignant bone tumor affecting children and adolescents.
Purpose of the Study:
- To investigate the role and mechanism of circ-CTNNB1 in osteosarcoma (OS) progression.
- To explore the interaction between circ-CTNNB1 and RNA binding motif protein 15 (RBM15).
- To elucidate the impact of circ-CTNNB1 on glycolysis and OS cell behavior.
Main Methods:
- Quantitative reverse transcription PCR (qRT-PCR) and Sanger sequencing to analyze circ-CTNNB1 expression.
- Biotin-labelled RNA pull-down, mass spectrometry, in vitro binding, and RNA electrophoretic mobility shift assays to study circ-CTNNB1-RBM15 interaction.
- In vitro and in vivo experiments to assess the functional impact of circ-CTNNB1 and RBM15 in OS cells.
Main Results:
- Circ-CTNNB1 was highly expressed in OS tissues and localized to the nucleus.
- Ectopic circ-CTNNB1 expression enhanced OS cell growth, invasion, and metastasis in vitro and in vivo.
- Circ-CTNNB1 interacted with RBM15, promoting glycolysis by upregulating HK2, GPI, and PGK1 via m6A modification.
Conclusions:
- Circ-CTNNB1 functions as an oncogene in osteosarcoma.
- Circ-CTNNB1 drives OS progression by promoting aerobic glycolysis through RBM15-mediated m6A modification.
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