Fluorescence Variation in Selective Sensing of Hg²⁺and Cu²⁺ Ions By Coumarin-xanthene Fused Optical Probe

The fluorescent moieties coumarin and xanthene (R6GCP) combined in a single molecule was designed and synthesized. The colorimetric and fluorescent variation of the probe towards the copper and mercury ions sensing is examined. With the added copper/mercury ions to the solution of R6GCP in DMF:H2O (2:8, v/v), the probe showed deep red color from yellow color. The probe showed turn-off and turn-on fluorescence for copper and mercury ion respectively. In the presence of other competing metal ions, the probe showed better sensitivity towards copper and mercury ions. The probe's detection limit found to be 5.29 × 10^-6 M and 1.24 × 10^-5 M for Cu²⁺ and Hg²⁺ ion respectively by the UV-visible spectral measurement. Fluorescence measurement, the detection limit for the Cu²⁺ and Hg²⁺ ions detection by this probe is 1.91 × 10^-7 M, and 1.32 × 10^-8 M respectively. 1:1 binding stoichiometry was confirmed between the probe and Cu²⁺/Hg²⁺ ions from jobs plot by UV-visible spectral technique. Moreover, R6GCP combined filter paper were prepared. These test paper containing probe could detect Cu²⁺/Hg²⁺ ions in real-time with a spontaneous color change.