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Transconjunctival Approach for Injection into the Rat Optic Nerve
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Clinically relevant small-molecule promotes nerve repair and visual function recovery.

Ngan Pan Bennett Au1, Gajendra Kumar1, Pallavi Asthana1

  • 1Department of Neuroscience, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong SAR.

NPJ Regenerative Medicine
|October 1, 2022
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Summary

Wolfberry extract (Lycium barbarum polysaccharides) promotes nerve regeneration in both the peripheral and central nervous systems. This study identified glycopyrrolate as a potential drug for enhancing nerve repair and restoring visual function after injury.

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Area of Science:

  • Neuroscience
  • Regenerative Medicine
  • Pharmacology

Background:

  • Mammalian injured axons exhibit limited regeneration in the central nervous system (CNS) compared to the peripheral nervous system (PNS).
  • Axon regeneration is crucial for functional recovery after nerve injury.

Purpose of the Study:

  • To investigate the potential of Lycium barbarum polysaccharides (LBP) in promoting axon regeneration after peripheral nerve injury (PNI) and optic nerve crush (ONC).
  • To identify FDA-approved small molecules that can enhance nerve repair and functional recovery.

Main Methods:

  • Administration of LBP in models of PNI and ONC.
  • Gene expression profiling of LBP-treated samples.
  • Querying the Connectivity Map database with LBP gene signatures.
  • Testing the identified small molecule, glycopyrrolate, in nerve injury models.

Main Results:

  • LBP significantly accelerated long-distance axon regeneration after PNI and ONC.
  • LBP promoted intrinsic neuronal growth capacity, RGC survival, and functional recovery.
  • Glycopyrrolate demonstrated efficacy in promoting PNS axon regeneration, RGC survival, and sustained CNS axon regeneration.
  • Glycopyrrolate enhanced visual function recovery after ONC.

Conclusions:

  • Lycium barbarum polysaccharides show significant potential for promoting axon regeneration and functional recovery in both PNS and CNS injuries.
  • Glycopyrrolate, an FDA-approved drug, can be repurposed for nerve repair, offering a promising therapeutic strategy for CNS injuries and visual function restoration.