Insight into the fraction variations of selenium and their effects on humification during composting

This study investigated the variation of selenium fractions and their effects on humification during composting. Selenite and selenate were added to a mixture of goat manure and wheat straw for composting. The results demonstrated that the bioavailable Se in the selenite added treatment (9.3-13.8%) was lower than in the selenate added treatment (18.1-47.3%). Meanwhile, the HA/FA of selenite and selenate added treatments were higher than in control, indicating that the selenium addition (especially selenite) promoted the humification of composting. Importantly, selenite enriched the abundance of Tepidimicrobium and Virgibacillus which were responsible to improve humification performance. Selenate increased the abundance of Thermobifida and Cellvibrio which facilitated the composting humification. The genes encoding CAZymes involved in the degradation of organic materials were also analyzed, and selenium could contribute to the synthesis of humus. KEGG pathway analysis revealed that the selenite addition promoted amino acids and carbohydrate metabolism compared to the control.