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Gene Therapy

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...

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Updated: Aug 27, 2025

Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

Published on: October 30, 2018

Fluorinated vectors for gene delivery.

Yu Wan¹, Yuhan Yang¹, Mingyu Wu¹

¹Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs, School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, China.

Expert Opinion on Drug Delivery

|October 2, 2022

Summary

This summary is machine-generated.

Fluorinated vectors offer improved gene delivery by overcoming limitations of traditional cationic vectors. Their unique properties enhance transfection efficiency and reduce cytotoxicity, paving the way for advanced gene therapy applications.

Keywords:

Gene delivery vectors fluorination modification polymer

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Area of Science:

Biomaterials Science
Gene Therapy
Nanotechnology

Background:

Traditional cationic gene delivery vectors often exhibit high cytotoxicity and poor serum stability, limiting their therapeutic efficacy.
Optimized gene delivery vectors are essential for advancing gene therapy and gene editing applications.
Fluorination presents a promising strategy to enhance vector performance due to unique physicochemical properties.

Approach:

This review explores the impact of fluorophilic effects on gene delivery efficiency.
It details chemical modification approaches for vector fluorination.
Recent advancements in fluorinated polymeric and lipidic vectors for gene therapy are summarized.

Key Points:

Fluorination imparts hydrophobicity, lipophobicity, and chemical/biological inertness to gene delivery vectors.
Fluorinated vectors demonstrate enhanced transfection efficiency and reduced cytotoxicity compared to conventional vectors.
Applications in gene therapy and gene editing are expanding with the development of these advanced materials.

Conclusions:

Fluorinated vectors are highly promising for gene delivery, though further research is needed for pure polymer synthesis, biosafety assurance, and mechanistic understanding.
Future applications could leverage fluorinated vectors' oxygen-carrying capacity, drug affinity, and imaging properties.
Continued development of fluorinated vectors will significantly impact the future of gene therapy and precision medicine.