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Personal size-separating impactor for sampling microbiological aerosols.

J M Macher, H C Hansson

    American Industrial Hygiene Association Journal
    |July 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

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    Researchers modified a personal impactor to collect viable airborne microorganisms on moist gelatin. This enhanced microbial sampling, improving recovery of dehydration-sensitive organisms compared to dry filters.

    Area of Science:

    • Environmental microbiology
    • Aerosol science
    • Instrumental analysis

    Background:

    • Personal impactors are crucial for assessing airborne microbial contamination.
    • Traditional impactors often use dry collection surfaces, potentially harming viable microorganisms.
    • Optimizing collection media is essential for accurate airborne microorganism sampling.

    Purpose of the Study:

    • To investigate the modification of a Marple personal cascade impactor for sampling viable microorganisms.
    • To evaluate the impact of a moist gelatin medium on the impactor's aerodynamic performance.
    • To compare the size-selective efficiency of the modified impactor with standard methods.

    Main Methods:

    • A Marple personal cascade impactor was adapted to use gelatin medium trays instead of filters.

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  • Aerodynamic particle sizer and the modified impactor were used to test particle cut-off diameters (D50).
  • Polydisperse aerosols and ambient bacterial/fungal aerosols were sampled to assess performance.

    • Main Results:

    • The modified impactor with gelatin showed minimal changes in aerodynamic cut-off diameters compared to Mylar filters.
    • Measured D50 values for stages four to seven ranged from 1.0 to 5.9 microns with gelatin.
    • Size separation efficiency of the modified sampler correlated well with the Andersen microbiological impactor.

    Conclusions:

    • Modifying personal impactors with moist gelatin media enhances the collection efficiency of viable airborne microorganisms.
    • This technique is particularly beneficial for sampling dehydration-sensitive bacteria and fungi.
    • The altered impactor maintains effective aerodynamic particle size selection for microbial aerosols.