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09:13
Isolation, Transfection, and Culture of Primary Human Monocytes
Published on: December 16, 2019
13.6K
UVA1 radiation attenuates pro-inflammatory functions in human monocytes
Anna Franziska Peters1,2, Yvonne Kusche1,2, Henrike Gerdkamp2
1Department of Dermatology, University of Münster, Münster, Germany.
The Journal of Dermatology
|October 3, 2022
Summary
UVA1 therapy impacts human monocytes, reducing their ability to fight infections and produce inflammatory signals. This study reveals a potential new anti-inflammatory mechanism for UVA1 in treating skin diseases.
Area of Science:
- Immunology
- Photomedicine
Background:
- UVA1 therapy effectively treats inflammatory and autoimmune skin diseases.
- The precise mechanisms of UVA1 action, particularly on innate immune cells like monocytes, remain unclear.
Purpose of the Study:
- To investigate the effects of UVA1 irradiation on the functional properties of human monocytes.
- To explore alterations in gene and protein expression profiles following UVA1 treatment.
Main Methods:
- Human peripheral blood monocytes were treated in vitro with 2 J/cm² UVA1 light.
- Functional assays and gene/protein expression analysis were performed after 48 hours of incubation.
Main Results:
- UVA1 treatment did not affect monocyte viability or apoptosis susceptibility.
- Monocyte phagocytosis and pathogen elimination (Leishmania major) capacities were reduced.
- Interleukin (IL)-1β mRNA production was significantly decreased in lipopolysaccharide-activated monocytes.
- UVA1-treated monocytes showed upregulated expression of the IL-1β decoy receptor.
Conclusions:
- UVA1 radiation interferes with fundamental monocyte functions, including phagocytosis, pathogen killing, and activation.
- UVA1 may attenuate pro-inflammatory IL-1 effects by reducing IL-1β production and upregulating its decoy receptor.
- This suggests a novel anti-inflammatory mechanism of UVA1 therapy in human monocytes.
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