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Morphological features of five neuronal classes in the gerbil lateral superior olive
Abstract:
Five morphologically distinct classes of neurons can be identified within the neuropil of the gerbil lateral superior olivary nucleus (LSO) by using a variety of histological techniques and electron microscopy. The physical features of these five classes resemble those found in the cat LSO and are identified, by using criteria and nomenclature established for the cat, as principal neurons, multiplanar neurons, marginal neurons, small neurons, and class 5 neurons. Principal cells compose approximately 75% of the total LSO neuronal population. They possess a discoid dendritic organization and are oriented rostrocaudally, perpendicular to the transverse curvatures of the LSO. Roughly 8% of the LSO population is composed of multiplanar neurons, whose dendritic fields are not restricted to any single plane of section. Both principal and multiplanar neurons share similar cytoplasmic features, and greater than 65% of their perikaryal surface is in contact with synaptic terminals. Small neurons compose approximately 11% of the LSO neurons, have the lowest percentage of their somal surface contacted by synaptic terminals (approximately 8%), and are found mostly in the middle/medial portions of the LSO. Marginal neurons, which compose approximately 6% of the LSO population, appear similar to principal neurons at the light microscopic level except that they are found along the contours of the LSO, oriented orthogonal to principal neurons. Approximately 28% of the somal surface of marginal neurons is in contact with synaptic terminals. The class 5 neuronal somata receive a similar number of axosomatic synaptic contacts as marginal neurons (approximately 31%) but are found well within the matrix of the LSO, aligned parallel to principal neurons. Class 5 neurons share the same light microscopic features as principal neurons and can be identified electron microscopically based only on the reduced percentage of somal surface occupied by synaptic terminals.