Determination of multidrug-resistant populations and molecular characterization of complex Klebsiella spp. in wild
Alessandra Tammy Hayakawa Ito de Sousa1, Marco Túlio Dos Santos Costa1, Stefhano Luis Cândido1
1Microbiology Laboratory of the Veterinary Hospital, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
Background And Aim:
One of the most significant public health concerns is multidrug-resistant (MDR) microorganisms. Klebsiella spp. have been at the forefront of causing different types of infections such as bacteremia, urinary tract infections, pneumonia, enteritis, and sepsis in humans as well as animals. This study aimed to determine the genomic similarity between Klebsiella spp. isolated from wild animal samples and those described in the Institut Pasteur genomic database to verify the spread of resistant clones regionally in the state of Mato Grosso, and to compare the epidemiological data in different regions of Brazil and the world.
Materials And Methods:
Isolates from various sites of injury in wild animals were identified by sequencing the 16S rRNA gene. Antimicrobial susceptibility testing was performed using the disk diffusion method to verify the resistance profile, and then, multilocus sequence typing was performed to verify the population structure and compare the isolates from other regions of Brazil and the world.
Results:
Twenty-three sequence types (STs) were observed; of these, 11 were new STs, as new alleles were detected. There was no predominant ST among the isolates. All isolates were MDR, with high rates of resistance to sulfonamides, ampicillin, amoxicillin, and nitrofurantoin and low resistance to meropenem, imipenem, and amikacin.
Conclusion:
Improving our understanding of the population structure of Klebsiella spp. in wild animals may help determine the source of infection during outbreaks in humans or animals, as the One Health concept emphasizes the interlinks between humans, animals, and environmental health.
Insights
Multidrug-resistant Klebsiella in wild animals shows high resistance to common antibiotics but remains susceptible to newer ones. Understanding their population structure is key to preventing human and animal infections.
Area of Science:
- Microbiology
- Genomics
- Public Health
Background:
- Multidrug-resistant (MDR) microorganisms pose a significant public health threat.
- Klebsiella spp. are common causes of infections in both humans and animals.
Purpose of the Study:
- To determine genomic similarity of Klebsiella from wild animals to known strains.
- To investigate the spread of resistant Klebsiella clones in Mato Grosso, Brazil.
- To compare epidemiological data globally.
Main Methods:
- 16S rRNA gene sequencing for isolate identification.
- Antimicrobial susceptibility testing using disk diffusion.
- Multilocus sequence typing (MLST) for population structure analysis.
Main Results:
- Twenty-three sequence types (STs) identified, including 11 novel STs.
- All isolates exhibited multidrug resistance.
- High resistance to sulfonamides, ampicillin, amoxicillin, and nitrofurantoin; low resistance to meropenem, imipenem, and amikacin.
Conclusions:
- Understanding Klebsiella population structure in wildlife aids in identifying infection sources.
- This knowledge supports the One Health approach, linking human, animal, and environmental health.
More Related Videos
09:39DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat VNTR - Fragment Length Analysis FLA
Published on: July 15, 2011
09:43Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
Published on: October 30, 2016
Related Concept Videos
01:29Modern Molecular Taxonomy
01:20Applications of Molecular Taxonomy