Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neuroimaging evidence for a dopamine-independent association between motor cortex microstructure and Parkinson's disease severity.

NPJ Parkinson's disease·2026
Same author

Lower synaptic density in mood circuitry underlies depression in Parkinson's disease.

Brain communications·2026
Same author

Optimized reference region and the effect on test-retest reliability and sensitivity to differences between Parkinson's disease and control groups with [<sup>11</sup>C]UCB-J.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2026
Same author

Synaptic density imaging in behavioral variant frontotemporal dementia: A comparison of <sup>1</sup> <sup>8</sup>F-SynVesT-1 and <sup>1</sup> <sup>8</sup>F-FDG PET.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Discovery of the First Brain-Penetrant Radioligands for Visualizing the GABA Transporter 1 (GAT-1): Synthesis, <i>In Vitro</i> Screening, and <i>In Vivo</i> Evaluation in Nonhuman Primates.

Journal of medicinal chemistry·2026
Same author

High-resolution in utero SV2A PET imaging of the nonhuman primate brain using the NeuroEXPLORER.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2026

Related Experiment Video

Updated: Aug 27, 2025

Determining Glucose Metabolism Kinetics Using 18F-FDG Micro-PET/CT
07:07

Determining Glucose Metabolism Kinetics Using 18F-FDG Micro-PET/CT

Published on: May 2, 2017

14.0K

Optimized Methodology for Reference Region and Image-Derived Input Function Kinetic Modeling in Preclinical PET.

Jason Bini1, Christine R Lattin2, Takuya Toyonaga1

  • 1Yale School of Medicine, New Haven, CT, USA.

IEEE Transactions on Radiation and Plasma Medical Sciences
|October 3, 2022
PubMed
Summary
This summary is machine-generated.

Optimizing preclinical PET imaging is crucial for novel radioligand development. This study recommends specific reconstruction algorithms and parameters for accurate kinetic modeling in small animals, potentially improving research efficiency.

Keywords:
attenuationkinetic modelingpositron emission tomography (PET)preclinicalquantificationreconstructionscattersmall animal

More Related Videos

Radiosynthesis, Quality Control, and Small Animal Positron Emission Tomography Imaging of 68Ga-Labelled Nano Molecules
09:55

Radiosynthesis, Quality Control, and Small Animal Positron Emission Tomography Imaging of 68Ga-Labelled Nano Molecules

Published on: October 4, 2024

519
Continuous Blood Sampling in Small Animal Positron Emission Tomography/Computed Tomography Enables the Measurement of the Arterial Input Function
10:21

Continuous Blood Sampling in Small Animal Positron Emission Tomography/Computed Tomography Enables the Measurement of the Arterial Input Function

Published on: August 8, 2019

8.4K

Related Experiment Videos

Last Updated: Aug 27, 2025

Determining Glucose Metabolism Kinetics Using 18F-FDG Micro-PET/CT
07:07

Determining Glucose Metabolism Kinetics Using 18F-FDG Micro-PET/CT

Published on: May 2, 2017

14.0K
Radiosynthesis, Quality Control, and Small Animal Positron Emission Tomography Imaging of 68Ga-Labelled Nano Molecules
09:55

Radiosynthesis, Quality Control, and Small Animal Positron Emission Tomography Imaging of 68Ga-Labelled Nano Molecules

Published on: October 4, 2024

519
Continuous Blood Sampling in Small Animal Positron Emission Tomography/Computed Tomography Enables the Measurement of the Arterial Input Function
10:21

Continuous Blood Sampling in Small Animal Positron Emission Tomography/Computed Tomography Enables the Measurement of the Arterial Input Function

Published on: August 8, 2019

8.4K

Area of Science:

  • Preclinical imaging
  • Nuclear medicine
  • Pharmacology

Background:

  • Small animal PET imaging is vital for biodistribution and pharmacology studies of novel radioligands.
  • PET acquisition and processing parameters can significantly influence kinetic modeling estimates.
  • The impact of these parameters on reference region and image-derived input function (IDIF) modeling is not well-established.

Purpose of the Study:

  • To investigate the effects of reconstruction algorithm, MAP iterations, MAP prior strength, and attenuation/scatter correction on small animal PET kinetic modeling.
  • To provide recommendations for optimizing preclinical PET methodology for novel radioligands.

Main Methods:

  • Dynamic PET/CT scans were conducted in house sparrows, rats, and mice using [11C]raclopride, [18F]AS2471907, and [11C]UCB-J, respectively.
  • Four factors were systematically evaluated: reconstruction algorithm (FBP vs. 3D-OSEM-MAP), number of MAP iterations, MAP prior strength (target resolution), and CT-based attenuation and scatter correction.

Main Results:

  • Filtered back projection (FBP) resulted in lower kinetic modeling estimates compared to 3D-OSEM-MAP reconstructions in sparrows and rats.
  • Higher target resolutions (0.8mm) are recommended, as lower resolutions (1.5-3.0mm) reduced kinetic parameters (e.g., V, BP) in rats and sparrows.
  • Omitting CT-based attenuation and scatter correction may be acceptable for short-lived isotopes in sparrows and mice to enhance throughput.

Conclusions:

  • Specific reconstruction algorithms and high target resolutions are essential for accurate small animal PET kinetic modeling.
  • The findings offer a framework for optimizing preclinical PET protocols, potentially improving the efficiency of novel radioligand development.
  • Consideration of omitting CT-based corrections can improve animal throughput without compromising data integrity in specific scenarios.