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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

812
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
812
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

468
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
468
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

722
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
722
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

502
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
502
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

309
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
309
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

510
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
510

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Related Experiment Video

Updated: Aug 27, 2025

Construction and Systematical Symmetric Studies of a Series of Supramolecular Clusters with Binary or Ternary Ammonium Triphenylacetates
06:35

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Recent Advances in Supramolecular Affinity Separations: Affinity Chromatography and Related Methods.

Ashley G Woolfork1, Sazia Iftekhar1, Susan Ovbude1

  • 1Department of Chemistry, University of Nebraska, Lincoln, NE 68588 (USA).

Advances in Chromatography
|October 3, 2022
PubMed
Summary
This summary is machine-generated.

Affinity chromatography utilizes supramolecular interactions for isolating specific targets. Recent advances enhance its application in biochemical purification, sample analysis, and chiral separations.

Keywords:
affinity chromatographyaffinity ligandsaffinity separationsaffinity supportsimmobilization methods

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Separation Science

Background:

  • Affinity chromatography is a key technique leveraging supramolecular interactions for target isolation.
  • It is widely used for measuring and characterizing specific molecules within complex biological samples.

Purpose of the Study:

  • To review fundamental concepts of affinity chromatography.
  • To examine recent advancements in affinity chromatography and related supramolecular separation techniques.

Main Methods:

  • Discussion of advances in support materials and immobilization strategies.
  • Analysis of novel binding agents used in affinity chromatography.
  • Review of supramolecular separation methods.

Main Results:

  • Summarizes new developments in affinity chromatography applications.
  • Highlights progress in support types, immobilization, and binding agents.
  • Details applications in biochemical purification, sample preparation, chiral separations, and biointeraction studies.

Conclusions:

  • Affinity chromatography continues to evolve with new materials and methods.
  • Recent developments expand its utility across various analytical and purification applications.
  • The technique remains crucial for detailed biointeraction studies and complex sample analysis.