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Recent Advances in Supramolecular Affinity Separations: Affinity Chromatography and Related Methods.
Ashley G Woolfork1, Sazia Iftekhar1, Susan Ovbude1
1Department of Chemistry, University of Nebraska, Lincoln, NE 68588 (USA).
Advances in Chromatography
|October 3, 2022
Summary
Affinity chromatography utilizes supramolecular interactions for isolating specific targets. Recent advances enhance its application in biochemical purification, sample analysis, and chiral separations.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Separation Science
Background:
- Affinity chromatography is a key technique leveraging supramolecular interactions for target isolation.
- It is widely used for measuring and characterizing specific molecules within complex biological samples.
Purpose of the Study:
- To review fundamental concepts of affinity chromatography.
- To examine recent advancements in affinity chromatography and related supramolecular separation techniques.
Main Methods:
- Discussion of advances in support materials and immobilization strategies.
- Analysis of novel binding agents used in affinity chromatography.
- Review of supramolecular separation methods.
Main Results:
- Summarizes new developments in affinity chromatography applications.
- Highlights progress in support types, immobilization, and binding agents.
- Details applications in biochemical purification, sample preparation, chiral separations, and biointeraction studies.
Conclusions:
- Affinity chromatography continues to evolve with new materials and methods.
- Recent developments expand its utility across various analytical and purification applications.
- The technique remains crucial for detailed biointeraction studies and complex sample analysis.
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