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Related Experiment Videos

Cl(-)-selective microelectrodes: sensitivity to anionic Cl- transport inhibitors.

A C Chao, W M Armstrong

    The American Journal of Physiology
    |August 1, 1987
    PubMed
    Summary

    Chloride-selective microelectrodes are affected by common transport inhibitors like furosemide, bumetanide, and SITS. These compounds interfere with accurate intracellular chloride measurements, impacting physiological studies.

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    Area of Science:

    • Physiological Measurement
    • Electrophysiology
    • Biomedical Engineering

    Background:

    • Chloride-selective microelectrodes are crucial for measuring intracellular chloride activity.
    • Anionic transport inhibitors like furosemide, bumetanide, and SITS are widely used in physiological research.
    • These inhibitors possess properties that suggest potential interference with microelectrode function.

    Purpose of the Study:

    • To investigate the interference of furosemide, bumetanide, and SITS with chloride-selective microelectrodes.
    • To quantify the extent of interference at physiological pH.
    • To assess the clinical relevance of these interferences for cellular studies.

    Main Methods:

    • Utilized double-barreled microelectrodes with Corning code 477315 liquid anion exchanger.

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  • Tested microelectrodes in solutions containing varying concentrations of furosemide, bumetanide, and SITS at pH 8.2 and physiological pH.
  • Measured microelectrode responses to chloride activity in the presence of these inhibitors.

    • Main Results:

    • Furosemide and bumetanide showed linear responses to their own logarithmic concentrations at pH 8.2.
    • At physiological pH, both furosemide and bumetanide (0.1 mM) significantly interfered with chloride measurements.
    • Microelectrodes exhibited approximately 150-fold higher sensitivity to furosemide and bumetanide than to chloride.
    • Sensitivity to SITS was approximately 1,000 times greater than to chloride.

    Conclusions:

    • Chloride-selective microelectrodes are significantly affected by common transport inhibitors furosemide, bumetanide, and SITS.
    • The interference is concentration-dependent and more pronounced at physiological pH.
    • Researchers must account for this interference when using these microelectrodes in studies involving these inhibitors to ensure accurate chloride activity measurements.