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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Aug 26, 2025

gP2S, an Information Management System for CryoEM Experiments

gP2S, an Information Management System for CryoEM Experiments

Published on: June 10, 2021

Practices for running a research-oriented shared cryo-EM facility.

Richard M Walsh^1,2, Megan L Mayer^1,2, Christopher H Sun^1,2

¹Harvard Cryo-EM Center for Structural Biology, Blavatnik Institute, Harvard Medical School, Boston, MA, United States.

Frontiers in Molecular Biosciences

|October 3, 2022

Summary

This model balances research productivity and user training in cryo-electron microscopy (cryo-EM). It streamlines sample preparation and data collection, enabling expertise development without delaying scientific breakthroughs.

Keywords:

cryo-EM on-the-fly pre-processing sample optimization shared facility user training

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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

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Last Updated: Aug 26, 2025

gP2S, an Information Management System for CryoEM Experiments

gP2S, an Information Management System for CryoEM Experiments

Published on: June 10, 2021

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

Area of Science:

Structural Biology
Microscopy

Background:

The Harvard Cryo-Electron Microscopy Center supports academic and commercial users.
Balancing research productivity with user training is a key challenge.

Purpose of the Study:

To present a model for operating a research-oriented cryo-electron microscopy facility.
To optimize training and research workflows.

Main Methods:

Implementing incremental training pipelines for cryo-sample preparation and data collection.
Offering staff-assisted screening and rapid access options for users.
Training users on Talos Arctica and Titan Krios microscopes for independent operation.

Main Results:

Overcoming bottlenecks in sample preparation through parallel skill development.
Enabling users to become expert electron microscopists.
Facilitating breakthroughs in research areas without compromising user expertise.

Conclusions:

The presented model effectively integrates research demands with user training.
This approach enhances productivity and fosters expertise in cryo-electron microscopy.