Bacitracin-Engineered BSA/ICG Nanocomplex with Enhanced Photothermal and Photodynamic Antibacterial Activity

To reduce the drug resistance of bacteria and enhance the antibacterial ability in bacterial infection therapy, we designed a new antibacterial nanoagent. In this system, a photosensitizer (indocyanine green, ICG) was loaded in bovine serum albumin (BSA) through hydrophobic-interaction-induced self-assembly to form stable BSA@ICG nanoparticles. Furthermore, a positively charged antibacterial peptide bacitracin (Bac) was physically immobilized onto the surface of BSA@ICG to generate a bacterial-targeted nanomedicine BSA@ICG@Bac through electrostatic interactions. Afterward, its photodynamic and photothermal activities were intensely evaluated. Moreover, its bactericidal efficiency was assessed via in vitro antibacterial assays and bacterial biofilm destruction tests. First, the obtained BSA@ICG@Bac showed both good singlet oxygen generation property and high photothermal conversion efficiency. In addition, it showed enhanced photodynamic and photothermal antibacterial capacities and biofilm-removing ability in vitro due to Bac modification. To sum up, our research provided an economic and less-time-consuming approach to preparing antibacterial nanomedicines with excellent antibacterial ability. Therefore, the prepared antibacterial nanomedicines have great potential to be utilized in clinical trials in the future.