A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

Area of Science:

• Oncology
• Molecular Biology
• Biochemistry

Background:

• Liquid biopsy (LB) analyzes tumor-derived molecules in bodily fluids, offering a minimally invasive alternative to traditional biopsies.
• LB is increasingly used in translational research and clinical oncology for early detection, monitoring, and resistance assessment.
• Reliable LB analysis hinges on meticulous preanalytical procedures, including sample collection, quality control, and storage.

Purpose of the Study:

• To present standardized protocols for collecting, processing, and storing plasma and serum samples for liquid biopsy.
• To ensure the reliability and reproducibility of liquid biopsy analyses based on circulating-free DNA.
• To provide flexible protocols applicable to various laboratory settings.

Main Methods:

• Development and presentation of standardized protocols for liquid biopsy sample handling.
• Focus on plasma and serum sample collection, processing, and storage.
• Protocols designed for circulating-free DNA analysis.

Main Results:

• Established standardized protocols for liquid biopsy sample preparation.
• Demonstrated the importance of preanalytical steps for downstream analysis.
• Protocols require standard laboratory equipment and are adaptable.

Conclusions:

• Standardized preanalytical protocols are essential for accurate and reproducible liquid biopsy results.
• The presented protocols facilitate reliable liquid biopsy analysis, particularly for circulating-free DNA.
• These guidelines support the clinical integration of liquid biopsy in cancer care.