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Related Experiment Video

Updated: Jun 15, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

Lipid Expansion Microscopy.

Brittany M White^1,2, Pratik Kumar³, Amanda N Conwell^1,2

¹Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, United States.

Journal of the American Chemical Society

|October 3, 2022

Summary

This summary is machine-generated.

Researchers developed lipid expansion microscopy (LExM) for super-resolution imaging of cellular membranes. This method precisely visualizes phospholipids and organelle membranes, overcoming light microscopy limitations.

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Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Published on: October 11, 2022

Area of Science:

Cell Biology
Microscopy
Biochemistry

Background:

Light microscopy is limited by diffraction, hindering visualization of nanoscale cellular structures like lipid bilayers.
Existing super-resolution techniques often require complex labeling or specialized equipment.

Purpose of the Study:

To develop a novel super-resolution imaging method for phospholipids within cellular membranes.
To overcome the diffraction limit for precise visualization of membrane structures.

Main Methods:

Developed lipid expansion microscopy (LExM), an all-small molecule approach.
Chemically anchored bioorthogonally labeled phospholipids into a hydrogel network.
Applied principles of expansion microscopy for enhanced resolution.

Main Results:

Achieved super-resolution imaging of metabolically labeled phospholipids in cellular membranes.
Precisely visualized organelle membranes, including nuclear invaginations.
Demonstrated compatibility with standard confocal microscopes.

Conclusions:

LExM enables high-precision super-resolution imaging of cellular membranes and phospholipids.
The method overcomes diffraction limitations for visualizing nanoscale membrane structures.
LExM offers broad applicability for studying various physiological contexts.