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GIFT: An ImageJ macro for automated fiber diameter quantification.

Jennifer Huling1, Andreas Götz1, Niels Grabow1

  • 1Institute for Biomedical Engineering, Rostock University Medical Center, Rostock, Germany.

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|October 3, 2022
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Summary

The GIFT macro offers automated, accurate fiber diameter measurement in electrospun materials using ImageJ. This user-friendly tool provides reproducible results and faster processing than existing software.

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Area of Science:

  • Materials Science
  • Biomedical Engineering
  • Nanotechnology

Background:

  • Accurate measurement of fiber diameters is crucial for characterizing electrospun materials.
  • Existing methods for fiber diameter analysis can be time-consuming and lack user-friendliness.

Purpose of the Study:

  • To develop and validate a user-friendly ImageJ macro for automated fiber diameter measurement in electrospun materials.
  • To provide a reproducible and efficient tool for researchers in the field.

Main Methods:

  • Development of the Graphical User Interface for Image Thresholding (GIFT) macro for ImageJ.
  • Application of a validated distance frequency-based method for fiber diameter analysis.
  • Comparison of GIFT macro performance against existing fiber diameter measurement software.

Main Results:

  • The GIFT macro demonstrated accurate and reproducible fiber diameter measurements.
  • The macro significantly reduced processing time per image compared to other software.
  • Performance was particularly strong for measuring very small fibers.

Conclusions:

  • The GIFT macro is a valuable, freely available tool for researchers analyzing electrospun materials.
  • It offers a user-friendly, efficient, and reliable solution for automated fiber diameter assessment.
  • The macro provides both raw data and averages for comprehensive analysis.