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Updated: Aug 26, 2025

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RBM3 interacts with Raptor to regulate autophagy and protect cardiomyocytes from ischemia-reperfusion-induced injury

Nan Wang1, Limeiting Wang1, Changyan Li1

  • 1Science and Technology Achievement Incubation Center, Kunming Medical University, Kunming, China.

Journal of Physiology and Biochemistry
|October 3, 2022
PubMed

Insights

RNA-binding motif protein RBM3 is upregulated during myocardial infarction. Downregulating RBM3 promotes apoptosis and inhibits autophagy in heart cells, highlighting RBM3

Area of Science:

  • Cardiology
  • Molecular Biology
  • Cell Biology

Background:

  • Acute myocardial infarction (AMI) presents significant global health challenges with unclear post-infarction mechanisms.
  • Identifying novel therapeutic targets for AMI is crucial for improving patient outcomes.
  • RNA-binding motif protein 3 (RBM3) is known for its roles in translation, tumor growth, and neuroprotection, but its function in myocardial infarction is largely unexplored.

Purpose of the Study:

  • To investigate the role and biological effects of RBM3 in the context of myocardial infarction.
  • To determine the impact of RBM3 on cardiomyocyte apoptosis and autophagy under ischemia-reperfusion conditions.
  • To elucidate the molecular mechanisms by which RBM3 influences myocardial injury.

Main Methods:

  • Assessing RBM3 expression levels in ischemia-reperfusion (I/R) models.
  • Manipulating RBM3 expression to observe effects on cardiomyocyte autophagy and apoptosis.
  • Investigating the interaction between RBM3 and Raptor to understand its role in regulating the autophagy pathway.

Main Results:

  • RBM3 expression was significantly increased under I/R conditions.
  • Downregulation of RBM3 led to the inhibition of autophagy and promotion of apoptosis in cardiomyocytes.
  • RBM3 was confirmed to interact with Raptor, modulating the autophagy pathway.

Conclusions:

  • RBM3 plays a protective role against ischemia-reperfusion-induced myocardial apoptosis.
  • The protective effects of RBM3 are mediated through the regulation of the autophagy pathway.
  • RBM3 represents a potential therapeutic target for mitigating myocardial injury following infarction.
Keywords:
Acute myocardial infarctionApoptosisAutophagyIschemia–reperfusionRNA-binding motif protein 3

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