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Updated: Aug 26, 2025
Author Spotlight: Eco-Friendly Extraction of Bioactive Compounds Using Polyol-Based Microwave-Assisted Techniques
Published on: August 23, 2024
Extraction optimization, biological activities, and application in O/W emulsion of deep eutectic solvents-based
Paula Virginia de Almeida Pontes1, Aline Czaikoski2, Naara Aparecida Almeida3
1Laboratory of Extraction, Applied Thermodynamics and Equilibrium (EXTRAE), Department of Food Engineering and Technology, School of Food Engineering, University of Campinas (UNICAMP), 13083-862, Campinas, São Paulo, Brazil.
Abstract:
This study evaluated the phenolic compound extraction from olive pomace with deep eutectic solvents (DES) prepared with choline chloride ([Ch]Cl) and four (poly-)carboxylic acids. Temperature, water addition in the solvent, and solid-liquid ratio were evaluate in total phenolic content and antioxidant activity of extracts obtained with DES and ethanol, as control. Moreover, the antimicrobial activities of solvents and extracts were evaluated. Oil-in-water emulsion with DES extract was prepared, characterized and its oxidative stability analyzed. The extract with the highest total phenolic content was obtained with [Ch]Cl:malonic acid. Under optimal conditions, DES extracted 9 % more total phenolic content than ethanol. DES extract showed superior antibacterial activity to the ethanolic extract, and its presence in oil-in-water emulsion increased the induction time in 10-fold when compared to the one prepared with water. These results reinforce that DES are a potential solvent for phenolic compound extraction from olive pomace with antibacterial and technological benefits.
