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The AKT/mTOR Signaling Pathway Was Mediated through the LINC00514/miR-28-5p/TRIM44 Axis

Lin Wu1, Xingmei Zhu2, Runfeng Wang3

  • 1Department of the Central Laboratory, The Second Affiliated Hospital of Xi'an Medical University, Xi'an 710038, China.

Disease Markers
|October 4, 2022
PubMed
Abstract

Insights

Long non-coding RNA LINC00514 promotes pituitary adenoma (PA) cell proliferation and invasion. LINC00514 acts as a ceRNA for miR-28-5p, impacting the AKT/mTOR pathway, suggesting it as a therapeutic target for PA.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Oncology

Background:

  • Long non-coding RNAs (lncRNAs) play crucial roles in tumorigenesis.
  • LINC00514 is implicated in various cancers, but its function in pituitary adenoma (PA) is unknown.

Purpose of the Study:

  • To investigate the role and molecular mechanism of LINC00514 in pituitary adenoma.
  • To evaluate LINC00514 as a potential therapeutic target for PA.

Main Methods:

  • LINC00514 expression was quantified using RT-qPCR in PA tissues.
  • PA cell proliferation and invasion were assessed after LINC00514 silencing using CCK-8 and transwell assays.
  • Molecular mechanisms involving ceRNA activity and signaling pathways were explored.

Main Results:

  • LINC00514 expression was significantly elevated in PA tissues.
  • LINC00514 silencing inhibited PA cell proliferation and invasion.
  • LINC00514 acts as a ceRNA for miR-28-5p, regulating TRIM44 and the AKT/mTOR pathway.

Conclusions:

  • LINC00514 promotes PA progression by regulating the LINC00514/miR-28-5p/TRIM44 axis and AKT/mTOR signaling.
  • LINC00514 represents a potential therapeutic target for pituitary adenoma treatment.

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