Related Experiment Video

Updated: Aug 26, 2025

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
07:55

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

8.1K

Locking down SOS Mutagenesis Repression in a Dynamic Pathogen

Leslie A Gregg-Jolly1

  • 1Department of Biology, Grinnell Collegegrid.256592.f, Grinnell, Iowa, USA.

Journal of Bacteriology
|October 4, 2022
PubMed

Insights

The DdrR coregulator helps control the SOS response in Acinetobacter baumannii. This finding offers new antimicrobial targets against this dangerous pathogen.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Acinetobacter baumannii is a significant ESKAPE pathogen known for its multidrug resistance.
  • The SOS response is a critical DNA damage repair mechanism in bacteria.
  • Understanding the regulation of the SOS response is crucial for developing new antimicrobial strategies.

Purpose of the Study:

  • To elucidate the role of the DdrR coregulator in the Acinetobacter baumannii SOS response.
  • To investigate how DdrR influences the repression of error-prone polymerases by UmuDAb.
  • To identify potential novel targets for antimicrobial therapies.

Main Methods:

  • Genetic analysis of the DdrR protein.
  • Biochemical assays to study protein interactions.
  • Assessment of bacterial survival under DNA-damaging conditions.

Main Results:

  • DdrR acts as a coregulator in the Acinetobacter baumannii mutagenic DNA damage response.
  • DdrR potentiates UmuDAb-mediated repression of error-prone polymerases.
  • This regulation impacts the bacteria's ability to repair damaged DNA.

Conclusions:

  • The DdrR coregulator plays a key role in modulating the SOS response in Acinetobacter baumannii.
  • Targeting the DdrR-UmuDAb interaction could be a viable strategy for developing new antibiotics.
  • Further research into the SOS response regulation may yield novel therapeutic approaches against A. baumannii infections.
Keywords:
AcinetobacterDdrRESKAPESOS mutagenesisSOS regulationUmuD

More Related Videos

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

10.8K
Following the Dynamics of Structural Variants in Experimentally Evolved Populations04:52

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Published on: February 3, 2023

1.0K

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.1K
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
5.1K
Mutations in Microorganisms01:18

Mutations in Microorganisms

Mutations are heritable changes in an organism’s genome involving alterations in the base sequence of DNA or RNA. These changes can influence cellular processes and phenotypic traits, potentially transforming the unaltered wild type into a mutant form. Such changes, termed forward mutations, are pivotal in shaping the genetic diversity of organisms.RNA viruses exhibit the highest mutation rates due to the absence of robust proofreading mechanisms during genome replication. In contrast,...
57
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.6K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.6K