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Updated: Aug 26, 2025

Comparing the Affinity of GTPase-binding Proteins using Competition Assays
Published on: October 8, 2015
GPR35, ally of the anti-ischemic ATPIF1-ATP synthase interaction
1Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50 - 40064 Ozzano Emilia, Bologna, Italy.
Abstract:
Mitochondrial ATP synthase synthesizes ATP for cellular functions; however, under various conditions, including ischemia, it hydrolyzes ATP, primarily to re-energize the mitochondria. ATP synthase inhibitory factor 1 (ATPIF1) inhibits hydrolysis of ATP by ATP synthase. Wyant and colleagues recently demonstrated that G-protein-coupled receptor 35 (GPR35) is involved in this process. This finding provides an additional framework for the novel discovery of potential therapeutic molecules against ischemia/reperfusion (I/R) injury.
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