A Multicalibration Urea Potentiometric Sensing Array Based on Au@urease Nanoparticles and Its Application in Home...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Nanotechnology

Background:

• Home potentiometric sensors offer real-time personal health monitoring but face reliability issues due to sample matrix variations (e.g., pH).
• Detection of urea in urine is a key application for home health devices, yet accuracy is often compromised by fluctuating sample conditions.

Purpose of the Study:

• To enhance the reliability of home potentiometric sensors for disease management.
• To develop a stable and accurate sensing system for urea detection in urine, adaptable to varying sample compositions.

Main Methods:

• Synthesized Au@urease nanoparticles to improve the stability of urease-based potentiometric sensors.
• Designed a multicalibrated urea potential (MCUP) sensing array comprising urea and pH electrode groups, each with sensing and calibration channels.
• Implemented a calibration mechanism within the MCUP array to correct for baseline potential shifts and pH-induced response slope variations.

Main Results:

• The Au@urease nanoparticles provided enhanced stability for the potentiometric sensor.
• The MCUP sensing array successfully calibrated the basic potential and response slope of the urea electrodes.
• The system demonstrated reliable urea detection in body fluids across a wide pH range.

Conclusions:

• The developed MCUP sensing array significantly improves the reliability of home potentiometric urea detection.
• This technology offers a robust solution for real-time health monitoring in diverse physiological conditions.
• The enzyme-based potentiometric array shows promise for application in various body fluid analyses.