Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Testes: Histology01:27

Testes: Histology

1.3K
A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
The spermatogenic cells, responsible for producing sperm, are...
1.3K
Spermatogenesis01:22

Spermatogenesis

3.6K
Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
3.6K
Development of the Sexual Organs in the Embryo and Fetus01:15

Development of the Sexual Organs in the Embryo and Fetus

1.2K
Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association of Toll-like receptor gene polymorphisms with susceptibility to hepatitis B virus-related acute-on-chronic liver failure: a case-control study.

Scientific reports·2026
Same author

Neoadjuvant chemoimmunotherapy versus neoadjuvant chemoradioimmunotherapy for locally advanced esophageal squamous cell carcinoma.

International journal of clinical oncology·2026
Same author

Accelerating tumor evolution and enhancing immunotherapy efficacy in lung adenocarcinoma based on EXO1 inhibition.

Translational lung cancer research·2026
Same author

Regional characteristics of esophageal cancer and robot-assisted minimally invasive esophagectomy (RAMIE): an analysis of the international UGIRA registry.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2026
Same author

Clinical efficacy of comprehensive traditional Chinese medicine in adjuvant therapy for stage Ib-IIIa resected non-small cell lung cancer: a multi-center, randomized, double-blind, placebo-controlled trial.

Frontiers in pharmacology·2026
Same author

Robotic-assisted bronchoscopy in dye localization in thoracoscopic pulmonary nodule resection: An initial experience.

JTCVS techniques·2026

Related Experiment Video

Updated: Aug 26, 2025

Germ Cell Transplantation and Testis Tissue Xenografting in Mice
10:41

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

Published on: February 6, 2012

29.9K

Immature rat testis sustained long-term development using an integrative model.

Yubo Ma1, Juan Chen1, Hecheng Li1

  • 1Department of Urology, The Second Affiliated Hospital of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, 710004, China.

Biological Research
|October 4, 2022
PubMed
Summary

This study developed an integrative model combining testicular culture and xenotransplantation. The model successfully maintained immature rat testicular tissue, achieving complete spermatogenesis and long-term survival in vivo.

Keywords:
Neonatal testisOrgan cultureOxidative stressSpermatogenesisXenotransplantation

More Related Videos

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

15.2K
In Vivo Microinjection and Electroporation of Mouse Testis
08:39

In Vivo Microinjection and Electroporation of Mouse Testis

Published on: August 23, 2014

28.2K

Related Experiment Videos

Last Updated: Aug 26, 2025

Germ Cell Transplantation and Testis Tissue Xenografting in Mice
10:41

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

Published on: February 6, 2012

29.9K
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

15.2K
In Vivo Microinjection and Electroporation of Mouse Testis
08:39

In Vivo Microinjection and Electroporation of Mouse Testis

Published on: August 23, 2014

28.2K

Area of Science:

  • Reproductive biology
  • Developmental biology
  • Xenotransplantation research

Background:

  • Traditional xenotransplantation for studying testicular development uses fresh tissue.
  • The suitability of cultured testicular tissue as a donor remains unclear.
  • An integrative model combining ex vivo culture and in vivo xenotransplantation was developed.

Purpose of the Study:

  • To explore the viability and developmental potential of immature rat testicular tissue using a novel integrative model.
  • To assess the survival and differentiation of xenografted testicular tissue.
  • To evaluate the functional and morphological outcomes of the integrative model.

Main Methods:

  • Neonatal rat testes (postnatal day 8) were cultured ex vivo for 4 days.
  • Cultured testes were xenotransplanted into castrated nude mice.
  • Grafts were analyzed after 57 days using histology, immunohistochemistry, immunofluorescence, TUNEL assay, gene, and protein expression analysis.
  • Comparison with a control group of rats harvested at postnatal day 69.

Main Results:

  • Complete spermatogenesis was established in xenografted testes.
  • Elevated expression of key testicular development and proliferation genes (e.g., DAZL, MKI67) was observed.
  • Evidence of oxidative stress (increased 8-OH-dG) and DNA methylation (DNMT3B) was detected.
  • Some seminiferous tubules showed thinner epithelium and vacuolation, with apoptotic germ cells present.

Conclusions:

  • The integrative model supports long-term survival and complete spermatogenesis of immature testicular tissue.
  • The model demonstrates potential for studying testicular development but requires optimization.
  • Oxidative damage may contribute to observed lesions, necessitating further investigation.