Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

6.7K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.7K
Disorders of Leukocytes01:27

Disorders of Leukocytes

1.1K
Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Context-dependent selection sculpts mutational landscapes in the normal lung.

Research square·2026
Same author

Transcriptional heterogeneity predicts and enables clonal selection in ageing haematopoiesis.

Research square·2026
Same author

Cathepsin B protease mediates high population density-induced mutagenesis to drive genome evolution and competitive growth.

Nature communications·2026
Same author

Selection for Function in Early Life: Implications for Early-Onset Pathologies.

Evolutionary applications·2026
Same author

Aberrant oxidative metabolism selects for <i>TET2</i> -deficient hematopoietic stem and progenitor cells.

bioRxiv : the preprint server for biology·2026
Same author

Hematopoietic stem cell aging promotes <i>TET2</i> clonal hematopoiesis.

Research square·2026

Related Experiment Video

Updated: Aug 26, 2025

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
10:33

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Published on: October 15, 2018

8.3K

Dangerous Liaisons between Tet2 Mutation, Inflammatory Monocytes, and Leukemogenesis.

Eric M Pietras1, James DeGregori1,2

  • 1Department of Medicine, Section of Hematology, University of Colorado Anschutz Medical Campus, Aurora, Colorado.

Cancer Discovery
|October 5, 2022
PubMed
Summary

Mice with a TET2 mutation showed faster aging-related myeloid leukemia and more inflammatory monocytes, suggesting a role in disease progression. This research offers insights into leukemia development and potential therapeutic targets.

More Related Videos

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line
06:38

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Published on: August 2, 2021

28.7K
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

10.0K

Related Experiment Videos

Last Updated: Aug 26, 2025

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
10:33

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Published on: October 15, 2018

8.3K
Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line
06:38

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Published on: August 2, 2021

28.7K
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

10.0K

Area of Science:

  • Hematology
  • Oncology
  • Immunology

Background:

  • The TET2 gene is crucial for regulating cell differentiation and is frequently mutated in myeloid malignancies.
  • Loss-of-function mutations in TET2 are associated with an increased risk of developing myelodysplastic syndromes and acute myeloid leukemia.

Discussion:

  • Transgenic mice expressing a common TET2 loss-of-function mutation recapitulated key aspects of human myeloid leukemia.
  • These mice displayed accelerated disease onset linked to aging.
  • A significant skewing of myelopoiesis towards the production of proinflammatory MHC-IIhi monocytes was observed.

Key Insights:

  • The TET2 mutation drives both accelerated leukemia development and a specific monocyte phenotype.
  • Proinflammatory MHC-IIhi monocytes may play a critical role in the pathogenesis of TET2-mutated myeloid leukemia.
  • This model provides a valuable tool for studying TET2-driven leukemogenesis and testing therapeutic interventions.

Outlook:

  • Further investigation into the functional role of MHC-IIhi monocytes in TET2-mutated leukemia is warranted.
  • Targeting these specific monocyte populations could offer novel therapeutic strategies.
  • Understanding the interplay between aging, TET2 mutations, and immune cell dysregulation is key for future research.