Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-Candida fungal cholecystitis - an uncommon occurrence even in the right host.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2026
Same author

Are you for real, <i>Tsukamurella</i>? Novel <i>Tsukamurella</i> species isolated from a patient with primary myelofibrosis.

ASM case reports·2026
Same author

Tough, Ductile, and Strong Hard-Soft Cementitious Composite Enabled by Multi-Material Additive Manufacturing.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

OmniCorr: an R-package for visualizing putative host-microbiome interactions using multi-omics data.

Bioinformatics advances·2026
Same author

Atherogenic index of plasma in stroke: A comprehensive review of its diagnostic, prognostic, and pathophysiological significance.

World journal of clinical cases·2026
Same author

Endocan as a Novel Biomarker for Endothelial Dysfunction and Cardiovascular Prognosis in ST-Elevation Myocardial Infarction: A Contemporary Literature Review.

Journal of personalized medicine·2026

Related Experiment Video

Updated: Aug 26, 2025

Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos
10:38

Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos

Published on: September 9, 2015

10.5K

Disulfiram Is Effective against Drug-Resistant Mycobacterium abscessus in a Zebrafish Embryo Infection Model.

Carlynn G Winters1, Ram M Basnet1, Paige E Faasuamalie1

  • 1Laboratory of Chronic Airway Infection, Pulmonary Branch, National Heart, Lung, and Blood Institute, Bethesda, Maryland, USA.

Antimicrobial Agents and Chemotherapy
|October 5, 2022
PubMed
Summary

Disulfiram shows potent activity against Mycobacterium abscessus infections in laboratory and animal models. This FDA-approved drug offers a promising new avenue for treating drug-susceptible and drug-resistant NTM infections.

Keywords:
Mycobacterium abscessusdisulfiramdrug resistantzebrafish

More Related Videos

Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery
10:19

Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery

Published on: June 26, 2014

13.3K
Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish
07:00

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Published on: October 8, 2018

10.8K

Related Experiment Videos

Last Updated: Aug 26, 2025

Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos
10:38

Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos

Published on: September 9, 2015

10.5K
Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery
10:19

Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery

Published on: June 26, 2014

13.3K
Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish
07:00

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Published on: October 8, 2018

10.8K

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Drug Discovery

Background:

  • Mycobacterium abscessus is an emerging nontuberculous mycobacterium (NTM) pathogen.
  • It poses a significant threat to individuals with cystic fibrosis (CF) and non-CF bronchiectasis.
  • Existing treatments for M. abscessus infections are often limited by drug resistance and toxicity.

Purpose of the Study:

  • To evaluate the efficacy of disulfiram, an FDA-approved drug, against Mycobacterium abscessus.
  • To assess disulfiram's activity against both drug-susceptible and drug-resistant M. abscessus strains.
  • To investigate disulfiram's potential for treating NTM infections in vivo.

Main Methods:

  • In vitro antimicrobial assays were performed against M. abscessus strains.
  • Intracellular macrophage assays were utilized to assess disulfiram's activity within host cells.
  • A zebrafish embryo infection model was employed to evaluate efficacy in a living organism.

Main Results:

  • Disulfiram demonstrated significant antimicrobial activity against drug-susceptible M. abscessus.
  • The drug was also effective against drug-resistant strains of M. abscessus.
  • In vivo studies in zebrafish embryos confirmed disulfiram's efficacy in treating M. abscessus infection.

Conclusions:

  • Disulfiram exhibits potent antimicrobial activity against Mycobacterium abscessus.
  • The findings support disulfiram as a potential therapeutic agent for NTM infections.
  • Further investigation into disulfiram for human NTM infections is strongly warranted.