Multi pathogenic microorganisms determination using DNA composites-encapsulated DNA silver nanocluster/graphene...

Area of Science:

• Nanotechnology
• Biotechnology
• Analytical Chemistry

Background:

• Accurate detection of pathogenic microorganisms is crucial for public health.
• Existing methods for multi-pathogen detection can be complex and time-consuming.
• Development of sensitive and selective sensing platforms is an ongoing challenge.

Purpose of the Study:

• To develop a novel DNA silver nanocluster (AgNCs)/graphene oxide (GO)-based system for the simultaneous determination of multiple pathogenic microorganisms.
• To utilize rolling circle amplification (RCA) for enhanced sensitivity in pathogen detection.
• To achieve a highly selective and efficient sensing platform for clinical applications.

Main Methods:

• Assembly of two different RCA-based DNA composites functionalized with DNA-stabilized AgNCs probes and ssDNA aptamers specific to target bacteria.
• Utilizing graphene oxide (GO) for capturing DNA composites and acting as a selective fluorescence quencher.
• Monitoring fluorescence signals generated upon target bacteria recognition and release of aptamers from GO.

Main Results:

• The developed platform successfully detected Escherichia coli and Staphylococcus aureus.
• Detection limits as low as 38 CFU/mL were achieved for both pathogens.
• The system demonstrated high selectivity and efficiency in distinguishing target bacteria.

Conclusions:

• The RCA/DNA-AgNCs/GO-based platform offers a promising approach for multi-pathogen determination.
• This method shows potential for applications in clinical diagnostics and therapy.
• The developed sensing platform is highly selective and efficient.