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Recent progress on small molecular temperature-sensitive fluorescent probes.

Yue Sun1, Manlin Fu1, Mianli Bian1

  • 1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China.

Biotechnology and Bioengineering
|October 6, 2022
PubMed
Summary
This summary is machine-generated.

Researchers reviewed organic small molecule temperature-sensitive fluorescence probes for detecting intracellular temperature. These noninvasive probes offer high sensitivity and selectivity, aiding the study of temperature-related biological processes in cells and animal models.

Keywords:
bio-imagingnoninvasivesmall moleculestemperature-sensitive fluorescence probes

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Area of Science:

  • Biophysics
  • Chemical Biology
  • Molecular Imaging

Background:

  • Intracellular temperature is a critical biophysical parameter linked to cellular metabolic activity.
  • Monitoring temperature changes within cells is vital for understanding temperature-dependent biological processes.
  • Fluorescence probes are emerging as powerful tools for noninvasive intracellular temperature detection due to their sensitivity and selectivity.

Purpose of the Study:

  • To review recent advancements in organic small molecule temperature-sensitive fluorescence probes.
  • To elucidate the common response mechanisms of these probes to temperature variations.
  • To highlight their practical applications in living cells and animal models, and discuss future clinical potential.

Main Methods:

  • Literature review of recent research on organic small molecule temperature-sensitive fluorescence probes.
  • Analysis of probe response mechanisms (e.g., fluorescence intensity, wavelength shifts).
  • Compilation of reported applications in cellular and in vivo models.

Main Results:

  • Organic small molecule probes demonstrate significant progress in sensitive and selective temperature detection.
  • Various response modes to temperature changes have been identified and utilized.
  • Successful applications in live cell imaging and small animal models have been demonstrated.

Conclusions:

  • Organic small molecule temperature-sensitive fluorescence probes are valuable tools for noninvasive temperature monitoring.
  • Further development holds promise for advancing our understanding of thermal biology and potential clinical diagnostics.
  • This review aims to demystify these probes and encourage broader research in this field.