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A Complex Methodological Approach for the Screening of Efficient and Safe Cobalt Chelators
Monika Moravcová1, Marcel Hrubša1, Zuzana Lomozová2
1The Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 50005 Hradec Králové, Czech Republic.
Medicinal Chemistry (Shariqah (United Arab Emirates))
|October 6, 2022
Summary
Researchers developed a new method to screen for safe and effective cobalt chelators. This approach helps evaluate potential treatments for cobalt toxicity from medical devices.
Area of Science:
- Biomedical Science
- Analytical Chemistry
- Toxicology
Background:
- Cobalt is essential but can cause toxicity from medical devices.
- No selective cobalt chelators are currently approved.
- Developing chelators is crucial for treating cobalt toxicity.
Purpose of the Study:
- To create a method for screening cobalt chelators.
- To evaluate the toxicity of cobalt-chelator complexes.
Main Methods:
- Standardized a spectrophotometric assay using NNDSA for cobalt chelation screening.
- Validated the assay with known metal chelators (EDTA, 8-hydroxyquinoline, chloroxine, nitroxoline).
- Assessed cobalt-chelator complex toxicity via Fenton reaction catalysis and erythrocyte haemolysis using HPLC.
Main Results:
- The NNDSA method detected 25-200 nM cobalt ions with high sensitivity.
- All tested chelator complexes reduced Fenton reaction rates.
- Chloroxine with cobalt caused significant erythrocyte lysis, unlike other compounds.
Conclusions:
- The developed method is a precise tool for evaluating cobalt chelators.
- It aids in identifying effective and safe treatments for cobalt toxicity.
- This approach supports the development of novel therapeutic options.
