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Model for ring closure in ER tubular network dynamics.

Ben Zucker1, Gonen Golani1, Michael M Kozlov1

  • 1Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.

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Summary

This study reveals how endoplasmic reticulum (ER) ring closure eliminates tubular networks. Feasible membrane tensions drive this process, explaining tubule and junction removal in cellular structures.

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Area of Science:

  • Cell Biology
  • Biophysics

Background:

  • Endoplasmic reticulum (ER) tubular networks are dynamic, with formation and elimination mechanisms.
  • Mechanisms for ER tubule and junction elimination were previously unknown.

Purpose of the Study:

  • To investigate the process of ER ring closure, which eliminates ER network elements.
  • To understand the role of membrane tension in ER network remodeling.

Main Methods:

  • Computational modeling of junctional knots with nanopores.
  • Analysis of membrane tension and its effect on pore sealing via fission.
  • Evaluation of hemi-fission as the rate-limiting step in pore sealing.

Main Results:

  • ER ring closure involves constriction into junctional knots and remodeling into regular junctions.
  • Gibbs' thermodynamic tension was considered as the driving force for ring closure.
  • Feasible membrane tensions were identified that facilitate spontaneous pore sealing.

Conclusions:

  • Membrane tension is a key factor driving ER ring closure.
  • The proposed model explains the elimination of ER network structures through feasible membrane tensions.