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Updated: Aug 26, 2025

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
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Potential Molecular Mechanism of Upregulated Aryl Hydrocarbon Receptor Nuclear Translocator 2 in Nasopharyngeal

Si-Wei Huang1, Gang Chen1, Jian-Di Li1

  • 1Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region 530031, China.

Computational and Mathematical Methods in Medicine
|October 7, 2022
PubMed
Abstract

Insights

Aryl hydrocarbon receptor nuclear translocator 2 (ARNT2) is overexpressed in nasopharyngeal carcinoma (NPC), suggesting it may be a key therapeutic target. This study explored ARNT2

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Nasopharyngeal carcinoma (NPC) therapy benefits are limited, necessitating novel therapeutic targets.
  • Aryl hydrocarbon receptor nuclear translocator 2 (ARNT2) role in NPC is underexplored despite its study in other cancers.

Purpose of the Study:

  • To investigate the expression level of ARNT2 in NPC.
  • To elucidate the underlying mechanisms of ARNT2 in NPC pathogenesis.

Main Methods:

  • Analysis of ARNT2 mRNA expression from public datasets.
  • Functional enrichment analysis of ARNT2-coexpressed and target genes.
  • ChIP-seq data analysis to identify ARNT2 regulatory targets.
  • Immune infiltration assessment using CIBERSORTx and correlation with ARNT2 expression.

Main Results:

  • ARNT2 is significantly upregulated in NPC with strong discriminatory power.
  • ARNT2 positively correlated genes are involved in cancer pathways; negatively correlated genes in immune pathways.
  • Prostaglandin-endoperoxide synthase 2 (PTGS2) identified as a potential ARNT2 target gene.
  • ARNT2 expression correlates with the infiltration of five immune cell types in NPC.

Conclusions:

  • ARNT2 overexpression in NPC may drive cancer progression, potentially via PTGS2 regulation.
  • ARNT2 represents a promising oncogenic target for NPC treatment.

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