Human regulatory T cells locally differentiate and are functionally heterogeneous within the inflamed arthritic joint

Tregs are crucial for immune regulation, and environment-driven adaptation of effector (e)Tregs is essential for local functioning. However, the extent of human Treg heterogeneity in inflammatory settings is unclear.

We combined single-cell RNA- and TCR-sequencing on Tregs derived from three to six patients with juvenile idiopathic arthritis (JIA) to investigate the functional heterogeneity of human synovial fluid (SF)-derived Tregs from inflamed joints. Confirmation and suppressive function of the identified Treg clusters was assessed by flow cytometry.

Four Treg clusters were identified; incoming, activated eTregs with either a dominant suppressive or cytotoxic profile, and GPR56⁺CD161⁺CXCL13⁺ Tregs. Pseudotime analysis showed differentiation towards either classical eTreg profiles or GPR56⁺CD161⁺CXCL13⁺ Tregs supported by TCR data. Despite its most differentiated phenotype, GPR56⁺CD161⁺CXCL13⁺ Tregs were shown to be suppressive. Furthermore, BATF was identified as an overarching eTreg regulator, with the novel Treg-associated regulon BHLHE40 driving differentiation towards GPR56⁺CD161⁺CXCL13⁺ Tregs, and JAZF1 towards classical eTregs.

Our study reveals a heterogeneous population of Tregs at the site of inflammation in JIA. SF Treg differentiate to a classical eTreg profile with a more dominant suppressive or cytotoxic profile that share a similar TCR repertoire, or towards GPR56⁺CD161⁺CXCL13⁺ Tregs with a more distinct TCR repertoire. Genes characterising GPR56⁺CD161⁺CXCL13⁺ Tregs were also mirrored in other T-cell subsets in both the tumor and the autoimmune setting. Finally, the identified key regulators driving SF Treg adaptation may be interesting targets for autoimmunity or tumor interventions.