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Updated: Aug 26, 2025
Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
Published on: November 6, 2021
An In vitro evaluation of effect of implant abutment on human gingival epithelial keratinocytes
Paulami Bagchi1, Ahmed Ali Alfawzan2, Swaroopkumar M Magar3
1Department of Prosthodontics Including Crown Bridge and Implantology, DY Patil Dental School, Lohegaon, Pune, Maharastra, India.
Background:
Abutment surfaces are being designed to promote gingival soft tissue attachment and integration. This confirms implant survival for long term by forming a seal around the prosthetics.
Objectives:
This study was done to compare the biocompatibility of three implant abutments: titanium uncoated, Ti-nitride coated, and modified polyetheretherketone (PEEK) with human gingival keratinocytes.
Materials And Methods:
The titanium-uncoated, titanium-nitride-coated, and modified PEEK discs (13 mm × 3 mm) were fabricated and compared with uncoated polyester cell culture discs, which were used as controls. These three implant abutments were evaluated for biocompatibility with respect to human gingival keratinocytes for viability, morphology, proliferation, and migration by scanning electron microscopy imaging and scratch wound healing assays. Measurements of roughness show changes between the investigated surfaces.
Results:
Keratinocytes cultured on all examined surfaces indicated adhesion and attachment. An assay of cell viability showed no substantial variances among the groups. The modified PEEK surface showed greater cell proliferation and migration among the three abutment materials.
Conclusion:
All three abutment material surface types showed similar epithelial biological responses. However, modified PEEK material showed the highest biocompatibility.
