Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

58.2K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
58.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Personalized targeting of BCL2 family proteins overcomes acquired resistance to BRAF-MEK inhibitors in preclinical melanoma.

Nature communications·2026
Same author

Equal Opportunity Disadvantage or Levels of Harm: Examining Differences in Collateral Consequences of the Sex Offender Registry by Race and Sexual Orientation.

Behavioral sciences & the law·2026
Same author

High-throughput multi-organ proteomics workflow for drug efficacy and toxicity analysis.

Nature communications·2026
Same author

Intratumoral bacteria are immunosuppressive and promote immunotherapy resistance in head and neck squamous cell carcinoma.

Nature cancer·2026
Same author

DrBioRight 2.0: an LLM-powered bioinformatics chatbot for large-scale cancer functional proteomics analysis.

Nature communications·2025
Same author

Ion suppression correction and normalization for non-targeted metabolomics.

Nature communications·2025

Related Experiment Video

Updated: Aug 26, 2025

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
12:22

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

Published on: January 22, 2013

33.7K

RPPA SPACE: an R package for normalization and quantitation of Reverse-Phase Protein Array data.

Huma Shehwana1, Shwetha V Kumar1, James M Melott1

  • 1Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Bioinformatics (Oxford, England)
|October 7, 2022
PubMed
Summary
This summary is machine-generated.

The new RPPA SPACE R package improves Reverse-Phase Protein Array (RPPA) data normalization by excluding poor-quality samples and introducing a novel noise metric for enhanced reliability.

More Related Videos

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

13.8K
A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

11.8K

Related Experiment Videos

Last Updated: Aug 26, 2025

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
12:22

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

Published on: January 22, 2013

33.7K
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

13.8K
A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

11.8K

Area of Science:

  • Proteomics
  • Bioinformatics
  • Computational Biology

Background:

  • Reverse-Phase Protein Array (RPPA) is a high-throughput, cost-effective method for quantitative protein measurement.
  • Normalization of raw RPPA data into analysis-ready formats presents a significant challenge.

Purpose of the Study:

  • Introduce RPPA SPACE, an R package designed to overcome RPPA data normalization challenges.
  • Provide a successor to the widely used SuperCurve software with substantial improvements.

Main Methods:

  • RPPA SPACE implements a novel normalization process that allows for the exclusion of poor-quality samples.
  • Introduces a 'noise' metric for quality control, estimating random errors on RPPA slides.
  • The package offers simpler input requirements and increased flexibility compared to SuperCurve.

Main Results:

  • RPPA SPACE enhances the quality of normalized data by removing unreliable samples.
  • The 'noise' metric aids in assessing the reliability and quality of RPPA slides.
  • RPPA SPACE demonstrates significantly improved speed and error reporting over SuperCurve.

Conclusions:

  • RPPA SPACE offers a robust, efficient, and user-friendly solution for RPPA data normalization.
  • The package improves data quality and reliability through advanced quality control metrics.
  • RPPA SPACE is a valuable tool for researchers utilizing RPPA technology in biological studies.