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Updated: Aug 26, 2025
Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells
Published on: March 16, 2022
Confocal Microscopy Studies of F-Actin Cytoskeleton Distribution and Dynamics Using Fluorescent LifeAct Constructs in
José Villanueva1, Yolanda Giménez-Molina2, Luis M Gutiérrez2
1Instituto de Neurociencias, Centro Mixto CSIC-Universidad Miguel Hernández, Alicante, Spain. jvillanueva@umh.es.
Abstract:
Cultured bovine chromaffin cells have been characterized as a successful model to study changes in the cytoskeleton during the secretory process. In this sense, the distribution and dynamics of the F-actin cytoskeleton can be studied by confocal microscopy using appropriate molecular tools such as LifeAct, a peptide that stains the structures of F-actin. In this work, we describe some methodological protocols making possible to study, under controlled stimulus conditions, the local dynamic changes of F-actin in the cortical zone and also to detect the simultaneous displacements of chromaffin granules and organelles in active zones.
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