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LncRNA MSC-AS1, as an oncogene in melanoma, promotes the proliferation and glutaminolysis by regulating the

Tian Tian1, Binjie Luo1, Guoliang Shen2

  • 1Department of Burn and Plastic Surgery, Taizhou People's Hospital, Taizhou.

Anti-Cancer Drugs
|October 7, 2022
PubMed

Insights

Long-noncoding RNA MSC-AS1 promotes aggressive melanoma growth by regulating the miR-330-3p/YAP1 pathway. Inhibiting MSC-AS1 suppressed tumor proliferation and glutaminolysis, offering a potential new melanoma treatment strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Melanoma is an aggressive skin cancer with high mortality.
  • Understanding the molecular mechanisms driving melanoma progression is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role and mechanism of long-noncoding RNA MSC antisense RNA 1 (MSC-AS1) in melanoma.
  • To explore the relationship between MSC-AS1, miR-330-3p, and YAP1 in melanoma cells.

Main Methods:

  • Quantitative real-time polymerase chain reaction to assess gene expression.
  • In vitro assays (cell count kit-8, clone formation, ELISA) to evaluate cell function.
  • Pull-down and luciferase reporter assays to validate molecular interactions.
  • In vivo xenograft model to assess tumor growth.

Main Results:

  • MSC-AS1 was upregulated in melanoma tissues and cells, correlating with poor prognosis.
  • MSC-AS1 promoted YAP1 expression by downregulating miR-330-3p, which directly binds to both.
  • Knockdown of MSC-AS1 suppressed melanoma cell proliferation and glutaminolysis, while YAP1 overexpression reversed these effects.
  • In vivo studies confirmed MSC-AS1 knockdown inhibited melanoma growth.

Conclusions:

  • MSC-AS1 facilitates melanoma cell proliferation and glutaminolysis via the miR-330-3p/YAP1 pathway.
  • MSC-AS1 represents a potential therapeutic target for melanoma treatment.

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